Proof of Concept "POC" Human Tumor Cell Lines

TD2 has more than 150 human tumor cell lines representing all of the major histologies as well as some rare tumors available for compound evaluation. Many lines have been characterized using gene expression profiling data that can guide cell line selection. Cell viability assays are available to quantify growth inhibitory effects of experimental agents, evaluate interactions between convention chemotherapy and experimental agents, and determine optimal drug sequencing. Both low and high-throughput screening is available.

SiRNA High-Throughput Evaluation

TD2 has a large siRNA library (sets include whole genome, druggable genome, kinome, and custom gene sets) that can be used to silence genes in the context of an agent and determine synthetic lethal partners. This is an invaluable resource to determine contexts that confer sensitivity and resistance, as well as the identification of rational combinations that will result in synergy.

TD2 has amassed a valuable set of informatics tools and methods for building knowledge networks from all available molecular information in order to gain insights into the relationships between genes, drugs, cellular processes and phenotypes. Knowledge mining can be incorporated into in vitro screens to provide additional information as to genomic contexts that correlate with sensitivity and resistance to agents.